Extended Data Fig. 9: Genetic knockdown of Ddx5 promotes chondrocytes apoptosis and inhibits chondrocytes proliferation in the early stages of mouse development. | Nature Aging

Extended Data Fig. 9: Genetic knockdown of Ddx5 promotes chondrocytes apoptosis and inhibits chondrocytes proliferation in the early stages of mouse development.

From: DDX5 inhibits hyaline cartilage fibrosis and degradation in osteoarthritis via alternative splicing and G-quadruplex unwinding

Extended Data Fig. 9

(a) In tissue sections taken 2 weeks after tamoxifen injection, knee joint sections of Ddx5fl/fl and Ddx5chon-/- mice were detected by SO&FG staining. COLX expression was detected by IF. The SO&FG staining showed significant shortening of the growth plate in Ddx5chon-/- mice, affecting both hypertrophic and non-hypertrophic regions, and causing a slight loss of articular cartilage. (b) PCNA expression was detected by IHC. Cell apoptosis was detected by TRUNEL kit (Scale bars, 100 μm). DDX5 expression was detected by IF (Scale bars, 200 μm). The blue and yellow lines in the panel represented the hypertrophic and non-hypertrophic cartilage thickness at the growth plate, respectively. The white or black dotted lines frame the cartilage or growth plate area.

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