Fig. 5: HK contains luteolin, which prevents stress-induced senescence. | Nature Aging

Fig. 5: HK contains luteolin, which prevents stress-induced senescence.

From: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice

Fig. 5

a, Top, Schematic representation of the experimental design; bottom, characterization of HK constituents by UPLC-QTOF-MS (Supplementary Table 4). b, Quantification of SA-β-Gal assay of UV-B-irradiated IMR90 fibroblasts, treated with the indicated compounds at 1 μM concentration or 10 μg ml−1 for HK (UV-B n = 9, isoquercetin n = 3, isorhamnetin n = 3, kaempferol n = 3, luteolin n = 7, luteolin-7-O-glucuronide n = 4, nobiletin n = 3, pinoresinol n = 4, rosmarinic acid n = 3, dicaffeoylquinic acid n = 4, HK n = 6 biological replicates). c, Quantification of SA-β-Gal-positive cells of UV-B irradiated IMR90 fibroblasts treated with different concentrations of Lut (0.01–2.5 μM) and HK (10 μg ml−1) (UV-B, Lut 0.01 μM, Lut 0.1 μM, n = 6 biological replicates; Lut 1 μM, Lut 2.5 μM, HK 10 μg ml−1, n = 3 biological replicates). d, Quantification of SA-β-Gal assay of Doxo-treated WI38 fibroblasts with or without Lut (0.1 and 1 µM) or HK (1 and 10 µg ml−1) (n = 2 biological replicates). e, Quantification of SA-β-Gal assay of Doxo-treated iCM cardiomyocytes treated with or without Lut (1 µM) or HK (100 µg ml−1) (n = 4 biological replicates). f, Luteolin plasma concentration in mice treated with a single dose of HK by oral gavage (0.5 mg kg−1) at 0, 15, 60 and 360 min (n = 3 animals per timepoint). g, Schematic representation of the experimental design. h, Representative pictures of SA-β-Gal staining (left) and quantification (right) on kidney sections. Insets show enlarged image of dashed box. (Untreated n = 3, Doxorubicin n = 2, Doxo + Luteolin n = 3 animals). Data in b, c, d, e, f and h are presented as mean ± s.e.m. Statistical test performed in b, c, d, e: one-way ANOVA with Dunnett’s multiple comparisons test. Statistical test performed in h: one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05; **P < 0.01, ***P < 0.001; ****P < 0.0001; NS, nonsignificant. Exact P values found in source data files. Image in Fig. 5a created in using BioRender.com.

Source data

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