Extended Data Fig. 1: Haenkenium expands healthspan and lifespan in mice and does not show toxicity in vivo.
a, Lifespan comparison of the most longest-lived mice from untreated and treated groups (top 50%); pink marks indicate females, blue marks indicate males (Female UT n = 11; Female T n = 14; Male UT n = 8; Male T n = 11). b, Survival curves for female mice (UT n = 21, T n = 27). c, Survival curves for male mice (UT n = 16, T n = 21) animals. d, Survival curves of animals hosted and treated in the different facilities (Facility 1 Treated n = 15; Untreated n = 15; Facility 2 Treated n = 23; Untreated n = 14; Facility 3 Treated n = 10; Untreated n = 8). e, Hematological parameters of treated and untreated animals: white blood cells (WBC), red blood cells (RBC), hemoglobin concentration (HGB), hematocrit (HCT) (WBC, HGB, HCT; UT n = 7, T n = 9. RBC, UT n = 6, T n = 8). f, Representative images of lung, kidney and liver of 24-month old treated and untreated animals after 4 months of treatment. No significant differences can be spotted among groups (photomicrographs representative of similar results from n = 4 mice per group). Scalebar: 200 μm. g, Glucose concentration after insulin injection (ITT upper panel) and after glucose injection (GTT, lower panel) (UT n = 7, T n = 9, Young n = 10). h, Average water intake during the whole day, ml/h (Young n = 15, UT n = 13, T n = 17). i-j, Lean and Fat mass were analyzed by EchoMRI in male (i) (UT n = 5. Treated n = 7. Young Control n = 4) and female (j) (UT n = 3, Treated n = 3, Young Control n = 4) animals. k, Physical appearance score progression of animals hosted and treated in the different facilities (Facility 1 Treated n = 15; Untreated n = 15; Facility 2 Treated n = 23; Untreated n = 14; Facility 3 Treated n = 10; Untreated n = 8). l, Area under the curve (AUC) analysis for data in e. (A.U. Arbitrary Units). m, Percentage of animals with kyphosis after 4 months of treatment (UT n = 27, T n = 46). n, Tumor prevalence after four months of treatment (UT n = 9, T n = 10). Data presented in panels e, g, h, i, j, k, l are presented as mean ± SEM. Statistical test used in b and c: Log-rank test. Statistical test used in e, l: Two-tailed unpaired t-test. Statistical test used in g: Two-way RM ANOVA with Dunnet’s multiple comparisons test. Statistical test used in h: One-Way ANOVA with Tukey’s test. Statistical test used in i, j: Two-way ANOVA with Dunnet’s multiple comparisons test. *=p < 0.05; ***=p < 0.001; # =p < 0.05 (UT vs Young Control). Exact p values found in source data files.
