Extended Data Fig. 6: TCA cycling metabolic flux analysis in cells.
From: Separation of reproductive decline from lifespan extension during methionine restriction
(a) 293 T, human embryonic kidney cell. ****P < 0.0001. (b) GC-1, mouse spermatogonia cell. ****P < 0.0001. (c) GC-2, mouse spermatocyte cell. ****P < 0.0001. n = 1×105 cells. n = 3 biological replicates. Regarding the determination of the flux, we employed a comprehensive approach, optimizing 10,000 simulated replicates and selecting the 50 simulated replicates (show the average) with the smallest discrepancy in the final target metabolite as the final solution set. Mean ± s.e.m. P values were obtained by unpaired, two-tailed t-test.
