Extended Data Fig. 3: Bone marrow monocytes/macrophages are more susceptible to senescence during aging.
a-b, The number of cells and the percentage of cells are displayed by cell frequency analysis. c, Dynamic changes of cell proportion in BMMs and neutrophils. d, UMAP plots show the distribution of senescence-related genes in different cell types in young and old bone marrow (BM-Y, BM-O). e, UMAP plots show the expression and distribution of senescence-related genes in old bone marrow cells (BM-O). f, SA-β-gal staining in BMMs isolated from young (3 m, n = 3 male mice) and aged mice (24 m, n = 3 male mice), respectively (scale bar, 50μm). g, The expression levels of senescence-related genes in BMMs (n = 3 mice). h, p53 immunofluorescence staining in BMMs (scale bar, 200μm; n = 3 mice; 5 ~ 6 images per n). i-j, Expression levels of senescence markers in liver, adipose tissue and brain of young mice after BMMs transplantation (n = 3 mice). k, Western blot analysis of p53 protein expression in muscle (n = 4 mice). l, Representative images of p21 immunohistochemistry in femur (scale bar, 50μm; n = 5 mice; 5 ~ 6 images per mouse). Mean ± SEM are shown for all panels. *P < 0.05; **P < 0.01; ***P < 0.001; #P < 0.0001 were determined using two-tailed t-test in a-f, g (Cdkn1a) and h, two-tailed t-test with a Welch’s correction in g (Cdkn2a) and one-way ANOVA followed by Tukey’s multiple comparison test in i-l.
