Extended Data Fig. 4: Aged follicular somatic cells elevate ROS levels and reduce mitochondrial membrane potential in young oocytes.
From: Rejuvenation of aged oocyte through exposure to young follicular microenvironment
a. Representative confocal images of cellular ROS stained with CM-H2DCFDA in oocytes from YY and YA RCFs. Scale bar, 100 μm. b. Quantification of CM-H2DCFDA fluorescence intensity in oocytes from YY and YA RCFs, as well as Y. n = 104 (Y), 122 (YY), 97 (YA). 2-month-old (young) and 14-month-old (aged) wide-type ICR mice were used. c. Fluorescence images of oocyte stained with MitoTracker Green (MTG, cyan) and mitochondrial membrane potential-sensitive dye TMRM (red). Scale bar, 100 μm. d. Quantification of the fluorescence intensity ratio of TMRM to MTG in oocytes from YY and YA RCFs, as well as Y. n = 110 (Y), 94 (YY), 72 (YA). 2-month-old (young) and 14-month-old (aged) wide-type ICR mice were used. Box plots in (b, d) show mean (black square), median (center line), quartiles (box limits), and 1.5× interquartile range (whiskers). One-way ANOVA, Tukey’s multiple comparisons test for (b, d). P value: ****P < 0.0001, ns, not significant (P > 0.05). The exact P values are presented in the Source Data. All data are from at least three independent experiments.
