Extended Data Fig. 5: Impact of young follicular somatic cells on aged oocyte quality.
From: Rejuvenation of aged oocyte through exposure to young follicular microenvironment
a, Quantification of oocyte death rates. Data are presented as mean ± SD. Sample sizes: n = 77 (YY), 65 (AA), 142 (AY) oocytes. Individual dots represent biological replicates for each group. Young: 2-3 months old, aged: 14-15 months old. b-e, Representative live-cell images (b) showing spindle and chromosomes in MII oocytes. Scale bar, 10 µm. Quantification of the percentage of chromosomal misalignment (c) and spindle abnormalities (d). Panel (e) presents a separate quantitative analysis of various classes of spindle abnormalities. Young: 2-3 months old, aged: 14-17 months old. f,g, Representative image of DAPI-stained blastocysts (f). Scale bars, 20 μm. Cell numbers per blastocyst were quantified in (g). n = 57 (YY), 43 (AY), 26 (AA). h-m, Comparison of various parameters between AA and AY RCFs and A: (h) cellular ROS levels, (i) oocyte maturation rates, (j) chromosomal misalignment, (k) spindle abnormalities, (l) blastocyst formation rate, and (m) blastocyst size. For (h), n = 72 (A), 72 (AA), 72 (AY); for (m), n = 25 (A), 28 (AA), 31 (AY). In (c, d, i, j, k, l), the oocyte numbers are specified in brackets. 2-month-old (young) and 14-month-old (aged) mice were used in (g-m). Box plots in (g, m) show mean (black square), median (center line), quartiles (box limits), and 1.5× interquartile range (whiskers). Box plots inside the violins in (h) show mean (black circle), quartiles (box limits), and 1.5× interquartile range (whiskers). One-way ANOVA with Tukey’s multiple comparisons test was used for (a, g, h, m). Two-tailed Fisher’s exact test for (c, d, i-l). P value: ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, ns, not significant (P > 0.05). Exact P values are in the Source Data. All data are from at least three independent experiments.
