Extended Data Fig. 6: TZP regeneration and oocytes transcriptomic remodeling in RCFs.
From: Rejuvenation of aged oocyte through exposure to young follicular microenvironment
a, Schematic demonstrating TZPs from GCs that pass through the zona pellucida, forming either adherens junctions or gap junctions on the oocyte surface. b, TZP regenerated within 3 hours of RCF culturing. RCF containing follicular somatic cells from mTmG mouse and wild-type oocytes were cultured within Alginate-rBM beads for 3 h. Somatic cells were then removed to visualize TZP regeneration. Scale bars, 20 μm. c, Histogram displays the number of up-regulated or down-regulated DEGs between oocytes from YY and AA, AY and AA, or YY and AY RCFs. d,e, Representative GO terms associated with the genes that were downregulated (d) and upregulated (e) in aged oocytes from AA RCFs when compared to young oocytes from YY RCFs. One-sided hypergeometric test with FDR adjustment for multiple comparisons.
