Extended Data Fig. 7: Investigating possible GC-to-oocyte mitochondrial transport in RCFs.
From: Rejuvenation of aged oocyte through exposure to young follicular microenvironment
a. Experimental design to study mitochondrial transport within RCFs. RCFs were created using somatic cells from transgenic MTS-mCherry-GFP1-11 mice, which express mitochondria-targeted mCherry, and unlabelled oocytes from wild-type mice. b. Confocal microscopy images of mCherry-labelled mitochondria in oocytes. Top panel: Positive control, an RCF formed by transplanting an MTS-mCherry-GFP1-11 oocyte into an MTS-mCherry-GFP1-10 r-follicle. Middle panel: RCF generated by transplanting a wild-type oocyte into an MTS-mCherry-GFP1-10 r-follicle. Bottom panel: Negative control, an RCF generated by transplanting a wild-type oocyte into a wild-type r-follicle. Rightmost panel of each row: overexposed images corresponding to the second column (mCherry). Somatic cells were partially removed before imaging to better observe oocyte fluorescence. Scale bar, 20 µm. All images are representative of at least three independent experiments.
