Extended Data Fig. 8: Comparative analysis of oocytes from YY, YA, AY, AA RCFs.
From: Rejuvenation of aged oocyte through exposure to young follicular microenvironment
This analysis examines various parameters of oocyte quality and developmental potential across four different RCF groups (YY, YA, AY, AA): (a) oocyte maturation rates (n = 12 YY, 4 YA, 10 AY, 9 AA), (b) chromosome misalignment (n = 9 YY, 4 YA, 5 AY, 5 AA), (c) spindle abnormalities (n = 9 YY, 4 YA, 5 AY, 5 AA), (d) blastocyst formation rates (n = 9 YY, 5 YA, 7 AY, 7 AA), (e) cellular ROS accumulation (n = 150 YY, 97 YA, 38 AY, 26 AA), (f) mitochondrial membrane potential (n = 153 YY, 72 YA, 57 AY, 53 AA). All metrics were normalized to those of the YY group in the same experiments using the non-normalized data as in Figs. 2h, j, k, l, 3b, d, 6f, j, Extended Data Fig. 4b, d, and 5c, d. The data were analyzed by one-way ANOVA, Tukey’s multiple comparisons test. P value: ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, ns, not significant (P > 0.05). The exact P values are presented in the Source Data. All results are presented as mean ± SD. All data are from at least three independent experiments.
