Extended Data Fig. 5: BFAR deficiency in CD8+ T cells from aged mice restored the aging-induced TRM decline.
From: Age-related decline in CD8+ tissue resident memory T cells compromises antitumor immunity
a, Schematic picture of BFAR gene targeting to generate CD8+ T cells conditional knockout mice (BFARCD8-KO) by crossing Bfar-floxed mice with Cd8-Cre mice. b, qPCR analysis of BFAR mRNA expression in naive CD4+ and CD8+ T cells isolated from WT and BFARCD8-KO mice. c-f, Flow cytometric analysis of the frequencies and absolute number of the indicated lymphoid immune cells in the thymus, spleen, and peripheral lymph nodes of WT and BFARCD8-KO mice. Data are presented as representative plots and summary bar graphs. DP, double positive; DN, double negative (n = 3 mice/group). g, qPCR analysis of Ifng and Gzmb mRNA expression in tumor-infiltrating CD8+ T cells from WT and BFARCD8-KO mice that were injected s.c. with MB49 tumor. h-k, Flow cytometric analysis (h,j) and the corresponding statistical analysis (i,k) of the frequencies of CD8+CD69+CD103+ TRM cells and IFNγ-producing CD8+ T cells of Rag1−/− mice that adoptively transferred with young/aged WT and BFAR-deficient splenic CD8+ T cells as indicated and then s.c. inoculated with MB49 tumor cells at the same day, or treated with α-CD103 antibody or control antibody (IgG) as indicated once every 3 days starting from day 7 (n = 6 mice/group). Bar graphs are presented as mean ± s.e.m. A two-tailed Student’s t-test was performed for comparisons. The data are representative of two (h-k) or three (a-g) independent experiments.
