Extended Data Fig. 1: Aging specifically impaired the generation of CD8+ TRM cells in NLTs.

a-d, Flow cytometric analysis of the frequencies and absolute numbers of CD4⁺TRM (CD103⁺CD69⁺), CD4⁺/CD8⁺ TCM (CD62L⁺CD44⁺) and CD4⁺/CD8⁺ TEM (CD62L⁻CD44⁺) cells in the liver and lung tissue of young (5-8-week-old) and aged (18-24-month-old) mice. Data are presented as representative plots (a, c) and summary graphs (b, d) (n = 3 mice/group). e-h, Flow cytometric analysis of the frequencies and absolute numbers of CD8⁺ TEM (CD62L⁻CD44⁺) and CD8⁺ TCM (CD62L⁺CD44⁺) cells (e,f), or flow cytometric analysis of the frequencies and absolute numbers of IFNγ-producing CD8⁺ TRM cells (g,h) of Rag1-KO mice that received young (2-month-old) or aged (18-month-old) mice-derived splenic CD8⁺ T cell and then were s.c. inoculated with MB49 tumor cells at the same day (n = 5 mice/group). i,j, Experimental scheme (i) and tumor growth (j) of Rag1-KO mice that were s.c. inoculated with MB49 tumor cells, and then intratumorally injected with equal numbers of tumor-infiltrating CD8⁺ TRM cells (10⁵/mice) that were isolated from young/aged tumor-bearing mice (n = 5 mice/group). Bar graphs are presented as mean ± s.e.m. A two-tailed Student’s t-test was performed for comparisons. The data are representative of two (i, j) or three independent experiments (a-h).

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