Extended Data Fig. 1: EBP1 is progressively decreased in Ebp1-CKO.
From: EBP1 potentiates amyloid β pathology by regulating γ-secretase
a, EBP1 RNA expression was analyzed using GEO datasets (GSE9770, GSE5281, and GSE36980) in human non-AD or AD brains (left, middle) and serial-aged brains (right). (left) Control: n = 71, AD: n = 34, (middle) Control: n = 47, AD: n = 32, (right) < 40: n = 12, 40-70: n = 9, 70-94: n = 16, 95-106: n = 4. b, Schematic strategy of CamkIIα-Cre; Ebp1flox/flox knockout mouse production. c, 2, and 6-month Ebp1-CKO and control mice brains were isolated, purified mRNA, and synthesized to cDNA. Comparative Ebp1 RNA expression in hippocampal and cortical tissues was analyzed with PCR. d, Ebp1-CKO and control mouse brains at serial ages (1, 4, 8, and 12 months) were applied to IHC with anti-EBP1 antibody (red) for detecting the decrease of progressive EBP1 protein expression. Nuclei were stained with DAPI. Quantification of EBP1 intensity in each region (bottom). Scale bar, 200 μm. n = 5. Data are presented as mean values ± SEM (a, d). *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001 (a, d), ns; not significant. Student’s two-tailed unpaired t-test (a) and Two-way ANOVA followed by Sidak’s multiple comparison (d).
