Extended Data Fig. 6: H2O2 orchestrates HDAC4 phosphorylation and its nuclear export.
a, Immunoblots (Left) and quantitative plot (Right) reveal HDAC4 unclear export, represented as an increased expression in the cytosol in response to intracellular H2O2 generated by DAAO in the presence of D-alanine (10 mM, for 72 h) in PECs (n = 4). b, p-HDAC4 immunostaining shows that intracellular H2O2 markedly increases HDAC4 phosphorylation that facilitates its translocation towards the cell cytosol in PECs (n = 6). Scale bar, 50 μm. Error bars represent SD (a). Data represent triplicated biologically independent experiments. P values were calculated using a two-tailed unpaired Student’s t-test (a). (*P < 0.05). Source data are provided as a Source Data file.
