Extended Data Fig. 8: Supplementary data from ex vivo optogenetic whole-cell patch-clamp recordings of PV and non-PV neurons in DTN.
a, Experiment design for injecting AAV-DIO-ChR2-EGFP into MVN of PV::Ai9 mice to compare the synaptic impact of PVMVN projection to PV and non-PV neurons in DTN via ex vivo optogenetics-coupled whole-cell patch-clamp recordings. b, A representative image showing the dense innervation of ChR2-expressing PVMVN terminals (green) in DTN (scale bar, 80 μm). c, Pairwise comparison of the amplitude of EPSC and IPSC recorded from the same PV (blue) and non-PV (grey) neurons upon photo-stimulation of PVMVN terminals in DTN. d-i, No statistically significant difference between PV and non-PV neurons was observed in other intrinsic and synaptic electrophysiological properties. Membrane resistance (d) and capacitance (e) of recorded PV (blue, n = 19) and non-PV (grey, n = 19) neurons. Latency (f), maximum rise slope (g), absolute peak voltage amplitude (h, left) and half-width (h, right) of optic-evoked EPSC recorded from PV (blue, n = 18) and non-PV (grey, n = 9) neurons in DTN upon blue-light stimulation of PVMVN axonal terminals. Absolute peak voltage amplitude (i, left) and half-width (i, right) of optic-evoked IPSCs recorded from PV (n = 18) and non-PV (n = 9) neurons in DTN upon blue-light stimulation of PVMVN axonal terminals. Volin plot displayed all sample points, median and quartiles of the data Bar charts presented data as mean ± s.e.m. Floating bars showed mean, minimal, and maximal value of the data.
