Fig. 3: The P-solubilizing activity of indigenous sewage bacteria as quantified by Single-Cell Raman Spectra.

SCRS (A) and the corresponding C-D ratio (B) of sewage bacteria incubated in the artificial culture media of MM-sP, MM-iiP, MM-oiP or MM-P_free with 50% D2O for 24 h, respectively. For comparison, also shown are SCRS (C) and the corresponding C-D ratio (D) of sewage bacteria incubated in dephosphorization sewage with 50%-D2O, either with no P (Sew-noP; as negative control) or supplemented with soluble P (Sew-sP), Ca3(PO4)2 (Sew-iiP) or lecithin (Sew-oiP) for 24 h, respectively. Sixty SCRS were acquired (one per cell) per condition, and all data points are presented here.