Table 1 Summary of sequencing results and diversity estimation metrics.

Depth (cm)

0–11.5

11.5–147

147–177

177–203

Number of samples

12

37

4

5

Number of total reads/Reads per sample

3,864,908/322,076 ± 111,640

6,047,561/163,448 ± 104,015

748,867/187,217 ± 96,097

1,049,518/209,904 ± 14,367

Total ASVs

3218

2453

1206

1331

ASVs > 0.1% of total

199

171

133

133

Shannon diversity index

5.46 ± 0.41

3.59 ± 0.94

3.88 ± 0.84

4.46 ± 0.55

Chao1

787 ± 322

183 ± 173

458 ± 334

518 ± 184

Inverse Simpson Index

110 ± 45

25 ± 16

21 ± 13

39 ± 20

Simpson Diversity Index

0.99 ± 0.01

0.92 ± .09

0.93 ± .06

0.96 ± .03