Fig. 4: H16 induces dose-dependent, large-scale Treg expansion and expression of biomarkers of differentiation and suppressive function in NHP.

a, b H16 administration to NHP drives Treg expansion in a dose-dependent manner. H16 was administered at 0 (vehicle only), 0.12 or 0.67 mg/kg to cynomolgus monkeys, and peripheral blood samples were collected at the indicated times. Multi-color flow cytometry was used to quantify (a) the percent of CD4+ Treg in WBC and CD4 T cells, and (b) cell counts for the CD4+ Treg, CD8 and NK cell populations by multiplying their frequency by the WBC counts. (c) H16 administration promotes the upregulation of biomarkers of CD4+ Treg differentiation and suppressive function. The median fluorescence intensity (MFI) as a function of time post-administration of H16 (0.12 mg/kg, orange; 0.67 mg/kg, purple) or vehicle (gray) is shown for CD25 (left panel), FoxP3 (middle panel), and Helios (right panel). Data are presented as mean ± SEM from three animals. CD4 cluster of differentiation 4, CD25 cluster of differentiation 25, FoxP3 forkhead box protein 3, MFI median fluorescence intensity, NHP non-human primate, NK natural killer, nonTreg non regulatory T cell, rhIL2 recombinant human interleukin-2, SEM standard error of mean, Treg regulatory T cell, WBC white blood cell.