Table 1 Comparison of human and microbial DNA recovery with and without host depletion
From: Unified metagenomic method for rapid detection of microorganisms in clinical samples
Sample number | Sample type | Routinely reported results | Depletion | Total number of read | Human Reads | Microbial reads | Target organisms |
---|---|---|---|---|---|---|---|
1 | BAL | P. aeruginosa (M) | No | 378,366 | 373,548 (99%) | 3377 (0.9%) | 2878 (0.8%) |
Yes | 213,695 | 41,635 (15%) | 152,418 (71%) | 140,544 (66%) | |||
2 | BAL | Upper respiratory tract flora | No | 145,961 | 145,926 (99.9%) | 2 | 2 |
Yes | 16,057 | 3594 (22%) | 7069 (44%) | 7069 (44%) | |||
3 | BAL | P. aeruginosa (S) | No | 36,343 | 35,778 (98%) | 368 (1%) | 187 (0.5%) |
Yes | 24,145 | 21,715 (90%) | 1224 (5%) | 579 (2.4%) |