Table 1 Comparison of human and microbial DNA recovery with and without host depletion

From: Unified metagenomic method for rapid detection of microorganisms in clinical samples

Sample number

Sample type

Routinely reported results

Depletion

Total number of read

Human Reads

Microbial reads

Target organisms

1

BAL

P. aeruginosa (M)

No

378,366

373,548 (99%)

3377 (0.9%)

2878 (0.8%)

Yes

213,695

41,635 (15%)

152,418 (71%)

140,544 (66%)

2

BAL

Upper respiratory tract flora

No

145,961

145,926 (99.9%)

2

2

Yes

16,057

3594 (22%)

7069 (44%)

7069 (44%)

3

BAL

P. aeruginosa (S)

No

36,343

35,778 (98%)

368 (1%)

187 (0.5%)

Yes

24,145

21,715 (90%)

1224 (5%)

579 (2.4%)