Fig. 6: Comparing human plaque T cell and myeloid cell subsets with mouse plaque T cells. | Nature Cardiovascular Research

Fig. 6: Comparing human plaque T cell and myeloid cell subsets with mouse plaque T cells.

From: Pairing of single-cell RNA analysis and T cell antigen receptor profiling indicates breakdown of T cell tolerance checkpoints in atherosclerosis

Fig. 6

a, Integration analysis of human plaque T cells and mouse plaque/aorta T cells. t-SNE plot shows T cells obtained from publicly available databanks: GSE131778 and GSE155512: human coronary and carotid plaques; GSE131776: aorta of Apoe−/− mice fed with a high-fat diet; GSE155513: the aorta of Apoe−/− and Ldlr−/− mice fed with high-fat diet; our data, aged Apoe−/− mice fed with chow diet. b, Comparison of all T cell subsets in aged WT and Apoe−/− mice and data from other public datasets. Cells from our data were colored; cells from other public databanks were labeled gray. T cell subsets were grouped to five T cell groups according to their functions in the t-SNE plot. Group I: CD4+ Teff/mem cells; group II: Treg cells; group III: CD8+ Teff/mem cells; group IV: naïve T cells; group V: γδ T cells. c, Percentages of CD8+ Teff/mem cells in human plaques, plaques of aged Apoe−/− mice, in aortas of high-fat-diet-fed Apoe−/− or Ldlr−/− mice at different ages. d, Myeloid cells obtained from the same databanks were integrated and analyzed. e, Comparison of all myeloid cell subsets in aged WT and Apoe−/− mice and data from other public datasets. Cells from our data were colored. Myeloid cell subsets were assembled into three major groups according to their functions in t-SNE analyses. Group I: macrophages, group II: DCs; group III: granulocytes. f, Percentages of macrophages in human plaques, in plaques of aged Apoe−/− mice, in aortas of high-fat-diet-fed Apoe−/− or Ldlr−/− mice at different ages. g, Heat maps show the similarities between human plaque T cell subsets and mouse plaque T cell subsets. The top 50 subset-specific genes were used to calculate the Spearman correlation coefficient between human and mouse T cell subsets. Mouse T cell subsets from four tissues were compared with human T cell subsets of six individuals. h, Dot plot displays tolerance-related genes in CD8+ Teff/mem cells and macrophages between human and mouse. Participants 1–4: human coronary plaques; participants 5–7: human carotid plaques (c and f–h).

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