Extended Data Fig. 1: Summary of the study findings.

Patients undergoing CABG had LV biopsies acquired on the beating heart. LV samples from donor hearts were included as controls. First, global alterations between CAD patients and control donor myocardium were assessed. a: Violin box-plot demonstrating the lower ATP/ADP ratio in CAD biopsies as compared to donor samples. b: Gene ontology analysis demonstrating upregulation of several biological processes associated with normal cellular function within ventricular cardiomyocytes of CAD patients compared to donor myocardium. Second, regional differences in LV metabolic profile were assessed, using paired biopsies from segments with and without inducible ischaemia (as determined by CMR). c: Principal component analysis of the liquid chromatography–mass spectrometry data revealing no clusters based on ischaemic (square) or remote (triangle) biopsies and no clear clusters of patients. d: Gene Ontology analysis demonstrating no significant differential expression of oxidative phosphorylation genes in ventricular cardiomyocytes between segments with or without inducible ischaemia. ADP = adenosine diphosphate; ATP = adenosine triphosphate; CABG = coronary artery bypass grafting; CAD = coronary artery disease; CMR = cardiovascular magnetic resonance; LV = left ventricular; vCM = ventricular cardiomyocyte.