Extended Data Fig. 5: Single-cell RNA sequencing (scRNA-seq) from atheromata. | Nature Cardiovascular Research

Extended Data Fig. 5: Single-cell RNA sequencing (scRNA-seq) from atheromata.

From: Loss-of-function mutations in Dnmt3a and Tet2 lead to accelerated atherosclerosis and concordant macrophage phenotypes

Extended Data Fig. 5

a, Flow cytometric analysis of chimerism in aortic single cell suspensions from mixed chimeric Ldlr−/− mice on 30 weeks of high fat, high cholesterol diet. Transplanted genotypes and ratios are indicated. Dot plots show live, doublet excluded, CD45 positive cells. b, Violin plots depict single-cell expression of key signature genes across the 5 classic mononuclear phagocyte populations. Color code follows Fig. 3b. c-e, Lesional cell distribution is shaped by genotype. (c) Major leukocyte lineages. Chi square test. (d) Monocyte subsets. Chi square test. (e) Proportions-of-clusters analysis within the lesional lymphocyte compartment. Displayed is log2 fold difference and 95% confidence interval. Permutation test with N = 1000 permutations. Full statistics are reported in Supplementary Table 3. f, Single-cell transcriptome-based cell cycle analysis in macrophages stratified by subset and genotype.

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