Extended Data Fig. 7: Expansion of Folr2+ Mrc1+ Ccl8+ Cxcl1+ macrophages in CHIP. | Nature Cardiovascular Research

Extended Data Fig. 7: Expansion of Folr2+ Mrc1+ Ccl8+ Cxcl1+ macrophages in CHIP.

From: Loss-of-function mutations in Dnmt3a and Tet2 lead to accelerated atherosclerosis and concordant macrophage phenotypes

Extended Data Fig. 7

a, UMAP plots of aligned gene expression data in single CD45.2+ cells isolated from aortae from WT; Vav1-Cre (n = 5015), Dnmt3a−/−; Vav1-Cre (n = 4499), and Tet2−/−; Vav1-Cre (n = 6078) populations (24-week cohort). b, Folr2+ Mrc1+ Ccl8+ Cxcl1+ (CHIP-TR) macrophages highlighted on the UMAP plots from a following 24 weeks of high-fat, high-cholesterol diet. Gray dots correspond to all other cells. Quantification in Fig. 4d. c, Gating strategy for identification of donor-derived tissue resident-like (TR) macrophages in atheromata by flow cytometry. The population was defined as CD45.2+ CD11b+ F4/80+ CD206hi/+. Comparison to other macrophages (CD206lo) confirmed higher expression of LYVE1 and FOLR2, and lower expression of CD9, as expected from scRNA-seq.

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