Extended Data Fig. 8: Multiplex Ion Beam Imaging by Time-of-Flight (MIBI-TOF) of aortic roots in WT, Dnmt3a−/− and Tet2−/−. | Nature Cardiovascular Research

Extended Data Fig. 8: Multiplex Ion Beam Imaging by Time-of-Flight (MIBI-TOF) of aortic roots in WT, Dnmt3a−/− and Tet2−/−.

From: Loss-of-function mutations in Dnmt3a and Tet2 lead to accelerated atherosclerosis and concordant macrophage phenotypes

Extended Data Fig. 8

a, MIBI-TOF of aortic roots in WT, Dnmt3a−/− and Tet2−/−. Shown are 3 exemplary antigens (in white) with relevance to atherosclerosis (von Willebrand factor [VWF], integrin subunit alpha X/CD11c and mannose receptor 1/CD206) out of 27 total markers recorded. DNA (blue) and SMA (magenta) channels are shown to provide anatomical reference. Scale bars = 200 μm. b, Biologically informed decision tree used to define cell identities (boxes) based on combinations of antigen markers. Black arrows signify condition fulfilled/marker positive. Red arrows signify that the preceding (combination of) condition(s) has not been fulfilled. & = AND. ∥ = OR. TR ΜΦ = tissue resident-like macrophage.

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