Extended Data Fig. 3: Flow cytometry analysis of macrophages and other immune cells.
a. Representative agarose gel images of PCR results using DNA extracted from the tail as a template in ATF3-CKO and WT littermate control mice. b. Relative mRNA expression of Atf3 in Mφ, CF, CM, and EC sorted from ATF3-CKO and WT mice (n = 6 mice per group). c. Representative IF images of ATF3 (green) and F4/80, Col1a2, CD31, and α-actinin (red) in the heart of ATF3-CKO and WT mice. Scale bar, 20 μm. This experiment was repeated independently triple with similar results. d. Representative flow cytometry plots of MerTK+ macrophages and the three clusters in ATF3-CKO and WT mice under sham and IR. e. Representative dot plots and quantification of neutrophils, T cells, and B cells in the heart of ATF3-CKO and WT mice post-IR (n = 6 mice per group). Statistical significance was evaluated using two-tailed unpaired t-test (b, e). All data are presented as mean ± SEM.
