Extended Data Fig. 7: Identification of cardiac resident macrophage-specific ATF3 deletion. | Nature Cardiovascular Research

Extended Data Fig. 7: Identification of cardiac resident macrophage-specific ATF3 deletion.

From: ATF3 coordinates the survival and proliferation of cardiac macrophages and protects against ischemia–reperfusion injury

Extended Data Fig. 7

a. Representative agarose gel images of PCR results using DNA extracted from the tail as a template in ATF3fl/flCx3cr1-cre+ and ATF3fl/flCx3cr1-cre- mice. b. Representative dot plots showing sorting strategy of CD45− cells, Cx3cr1− macrophages, and Cx3cr1+ macrophages. Relative mRNA expression of Atf3 in three types of cells in two genotypes (n = 6 mice per group). c. Representative IF images of F4/80 (green), ATF3 (red) and CX3CR1 (pink) in the hearts of the two genotypes. Scale bar, 20 μm. Statistical significance was evaluated using two-tailed unpaired t tests (b). All data are presented as mean ± SEM.

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