Extended Data Fig. 9: Effect of ATF3 deficient MerTK+ macrophage transfer on cardiac repair post IR. | Nature Cardiovascular Research

Extended Data Fig. 9: Effect of ATF3 deficient MerTK+ macrophage transfer on cardiac repair post IR.

From: ATF3 coordinates the survival and proliferation of cardiac macrophages and protects against ischemia–reperfusion injury

Extended Data Fig. 9

a. Scheme showing WT-MerTK+ and ATF3 deficient-MerTK+ macrophages transferred into ATF3-CKO mice. b, c. Flow cytometry analysis of Annexin V+ cells (b) and EdU+ cells (c) gated on Calcein-AM+MerTK+ macrophages in the heart of mice transferred with two types of MerTK+ macrophages (n = 6 mice per group). d–g. Immunostaining (d) and flow cytometry analysis (e) of CD31+ cells in the cardiac border area, EF (f), and cardiac fibrosis (g) in the heart of ATF3-CKO mice injected with WT-MerTK+ and ATF3 deficient-MerTK+ macrophages (n = 6 mice per group). Statistical significance was evaluated using two-tailed unpaired t-test (b–g). All data are presented as mean ± SEM.

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