Extended Data Fig. 1: Identification of macrophage/monocyte subpopulations.
a. Uniform manifold approximation and projection (UMAP) of single-cell RNA-sequencing (scRNA-seq) revealed 11 unique clusters (left) and the percentage of cells per cell type (right) in the heart between sham and IR. Mφ, macrophage; Mon, monocyte; CF, cardiac fibroblast; Neu, neutrophil; DC, dendritic cell; EC, endothelial cell; SMC, smooth muscle cell; CM, cardiomyocyte; LPC, lymphoid progenitor cell. b. Heatmap of the top ten differentially expressed genes (DEGs) in seven clusters of macrophages/monocytes. c. Hierarchical clustering of macrophages and monocytes. d. Violin plots representing log-normalized expression of Mertk and Ccr2 in the seven macrophage/monocyte clusters. e. Violin plots representing the log-normalized expression of Timd4, Folr2, and Cd163 in MerTK+CCR2- macrophages and MerTK−CCR2+ macrophages. f, g. Colocalization (up) and quantification (down) of immunofluorescence (IF) signals of F4/80 and LYVE1, TREM2, and CD74 in cardiac border (f) and remote (g) regions of the sham and IR (n = 6 mice per group). Scale bar, 20 μm. HPF, high-power field. Statistical significance was evaluated using two-tailed unpaired t tests (f and g). All data are presented as mean ± SEM.
