Extended Data Fig. 4: Loss of BRD4 in Mef2c-AHF-Cre-SHF CPCs in vivo results in right ventricular thinning.
From: A genome-wide CRISPR screen identifies BRD4 as a regulator of cardiomyocyte differentiation
(a) Hematoxylin and eosin staining of a section through outflow tract and RV of Mef2c-AHF-Cre;Brd4flox/+ and Mef2c-AHF-Cre;Brd4flox/flox embryos at E13.5 and quantitative assessment of right ventricular myocardial wall thickness in indicated genotypes (19 sections from n = 3 control embryos and 18 sections from n = 3 mutant embryos). (b) Quantification of percentage phospho-histone H3- and cleaved caspase 3-positive cells in the RV of E13.5 Mef2c-AHF-Cre;Brd4flox/+ and Mef2c-AHF-Cre;Brd4flox/flox embryos (n = 3-4 biologically independent samples per genotype). (c) Hematoxylin and eosin staining of a section through outflow tract and RV of Mef2c-AHF-Cre;Brd4flox/+ and Mef2c-AHF-Cre;Brd4flox/flox embryos at E10.5 and quantitative assessment of right ventricular myocardial wall thickness in indicated genotypes (6 sections from n = 3 control embryos and 6 sections from n = 3 mutant embryos). (d) Quantification of percentage phospho-histone H3- and cleaved caspase 3-positive cells in the RV of E10.5 Mef2c-AHF-Cre;Brd4flox/+ and Mef2c-AHF-Cre;Brd4flox/flox embryos (n = 3 biologically independent samples per genotype). Error bars represent ±1 SEM. All comparisons are made as indicated; * represents p = 0.0489, ** represents p = 0.0146, **** represents p < 0.0001 (two-tailed unpaired t test). RV, right ventricle; LV, left ventricle. Scale Bars = 100 µm (a, c).
