Extended Data Fig. 5: AAV-sR3 induced dLV regression causes only modest changes in the behavioral phenotype of WT and APdE9 mice.
a-z, Comparison of behavioral results in littermate male AAV-Ctrl treated WT (n = 10), APdE9 (n = 11) and AAV-sR3 treated WT (n = 12) and APdE9 (n = 9) mice at 13-14 months of age. NB, nest building; LD, Light–dark; OF, Open field; MWM, Morris water maze; BM, Barnes maze; FC, Fear conditioning. a, Experimental schedule of all behavioral tests. b-d, NB results showing (b) nest scores at timepoint 1 (c) nest scores at timepoint 2, and (d) amount of unshredded cotton at the end of both time points. e-h, LD results showing total (e) distance traveled in light side (f) distance traveled in dark side (g) distance traveled, and (h) time spent on light side. i-j, OF results showing total (i) time spent at the center of open field arena and (j) distance moved in open field arena. k-n, MWM results showing (k) escape latency in eight training sessions (S1-S8; total four days with two training sessions per day). (l) escape latency in last training session (S8, reverse training), (m) total time spent in trained quadrant in probe trial, and (n) total time spent in trained platform in probe trial. o-t, BM experimental results showing (o, r) latency to target zone in probe trials 1-2, (p, s) time spent in target zone in probe trials 1-2, (q) time spent at the center of arena in probe trial 1, and (t) time spent in target and adjacent zones of the arena in probe trial 2. u-z, FC results at day 1 and 2 showing (u, x) freezing % during 180 seconds, (v, y) total distance moved, and (w, z) mean explored area %. Data shown are representative of a single experiment using littermate mice. Datapoints in graphs represent individual mice. The NB, MWM, BM and FC illustrations were created with BioRender.com. P values were calculated using (e-j, l-z) two-way ANOVA, (b-d) two-way repeated measures ANOVA, and (b-d, k) three-way repeated measures ANOVA all with Tukey’s post hoc test for multiple comparison. Data are presented as mean values ± s.e.m.
