Extended Data Fig. 7: AAV-VC induced dLV expansion in APdE9 and 5xFAD mice does not affect LN, spleen, or body weight.
a, Experimental schedule for panels b–p; comparison of littermate AAV-Mock and AAV-VC-treated i.c.m. (male) and i.c.v. (female) injected WT and APdE9 mice at 9 months of age. b, Simplified schematic illustration of dLVs (green) attached to the basal cranium and spinal canal after removal of the brain and spinal cord. c-h, Comparison of dLVs (white) in (c-d) PPA and (e-h) CNII region of i.c.v. (n = 3,3,3,3) and i.c.m. (n = 4,3,3,3) injected APdE9 and WT mice. i-l, Comparison of (i) body (n = 23,23,13,15), (j) dcLN (n = 23,23,13,15), (k) scLN (n = 21,22,10,13), and (l) spleen weight (n = 23,23,13,15) in i.c.v. injected APdE9 and WT mice. m-p, Comparison of (m) body (n = 20,19,18,20), (n) dcLN (n = 16,15,14,16), (o) scLN (n = 20,18,18,20), and (p) spleen weight (n = 20,19,18,20) in i.c.m. injected APdE9 and WT mice. q, Experimental schedule for panels r-v; comparison of littermate AAV-Mock and AAV-VC-treated i.c.m. (male and female) and i.c.v. (male) injected WT and 5xFAD mice at 4.5 months of age. r-s, Comparison of (r) body (n = 13,13,11,12) and (s) dcLN (n = 9,9,7,8) weight in i.c.v. injected 5xFAD and WT mice. t-v, Comparison of (t) body (n = 8,8,6,7), (u) dcLN (n = 11,11,7,8), and (v) scLN (n = 15,15,11,12) in i.c.m. injected 5xFAD and WT mice. Data shown are representative of at least two independent experiments using littermate mice. Datapoints in graphs represent individual mice. dcLN weights (j, n, r, u) represent an average of both sides (left and right, max one LN per side per mouse) and scLN weights (k, o, v) represent an average of all LNs on the left side of the body. P values were calculated with (e, f, j-l, n-p, s, u, v) two-way ANOVA, (r, t) three-way repeated measures ANOVA, and (i, m) three-way repeated measures mixed-effects model with Tukey’s post hoc test for multiple comparison. Data are presented as mean values ± s.e.m. Scale bars: 100 µm (g, h) and 200 µm (c, d).
