Fig. 5: AAV-sR3 induced dLV regression does not affect brain Aβ load in 5xFAD mice.
Comparison of littermate AAV-Ctrl- and AAV-sR3-treated WT and 5xFAD male mice at 4.5 months of age. a, Schedule indicating AAV administration and experimental analysis time points. b, Schematic illustration indicating brain quantification areas in panels (c–p). c,j, Representative images of D54D2 Aβ staining (red) in HC (c) and cortex (j) region. Example of a quantified HC area without dSBC is outlined with the white dashed line. d–p, Quantification of Aβ staining in HC (WO2; n = 13 and 13 and D54D2; n = 14 and 11) (d–i) and cortex (WO2; n = 13 and 13 and D54D2; n = 14 and 12) (k–p) region. q–v, Comparison of body weight (q), grid hanging (r), open field (s) and elevated plus maze (EPM) (t–v) results (n = 13, 12, 14 and 13). Data shown are representative of at least two independent experiments using littermate mice. Data points shown in graphs represent individual mice. Brain Aβ values represent an average of six brain sections (400 µm apart) per mouse and are normalized to average of 5xFAD-Ctrl group of every experimental set. P values were calculated using unpaired two-tailed t-test (d–i,k–p) and two-way ANOVA with Tukey’s post hoc test (q–v) for multiple comparisons. Data are presented as mean ± s.e.m. Scale bars, 500 µm (c,j).
