Extended Data Fig. 3: Ablation of the PD-1/PD-L1 pathway impairs neonatal heart regeneration.
a-c, Ejection fraction (a), left ventricular end-diastolic volume (b) and left ventricular end-systolic volume (c) of IgG- or anti-PD-L1-treated mice at 1, 2, and 3 weeks after P3-MI. IgG sham: n = 6, 5, and 5 mice at 1, 2, 3 weeks, respectively. anti-PD-L1 sham: n = 6 mice. IgG MI: n = 5, 6, and 6 mice at 1, 2, 3 weeks, respectively. anti-PD-L1 MI: n = 7 mice (a, b c). p = 0.00052 (1 week), p = 0.000008 (2 weeks), p = 0.000009 (3 weeks) (a). p = 0.004638 (2 weeks), p = 0.000823 (3 weeks) (b). p = 0.002964 (2 weeks), p = 0.000145 (3 weeks) (c). d-f, Ejection fraction (d), left ventricular end-diastolic volume (e) and left ventricular end-systolic volume (f) of Pd-1+/− and Pd-1−/− mice at 1, 2, and 3 weeks after P2-MI. Pd-1+/− sham: n = 5, 5, and 4 mice at 1, 2, 3 weeks, respectively. Pd-1−/− sham: n = 5 mice. Pd-1+/− MI: n = 4 mice. Pd-1−/− MI: n = 6, 5, and 5 mice at 1, 2, 3 weeks, respectively (d, e, f). p = 0.000703 (1 week), p = 0.001559 (2 weeks), p = 0.021383 (3 weeks) (d). p = 0.01633 (2 weeks), p = 0.019505 (3 weeks) (e). p = 0.004214 (2 weeks), p = 0.042845 (3 weeks) (f). The same cohort of mice was measured every week. Results are shown as mean ± s.e.m; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by two-tailed Student’s t-test.
