Fig. 1: Development of mouse models for inducible APOB lipoprotein dyslipidemia.
From: Kupffer cells dictate hepatic responses to the atherogenic dyslipidemic insult
a, Plasma lipid levels 10 d after tamoxifen administration in APOE cKO (green bars) and D374Y (blue bars) strains together with respective littermate controls in male and female mice fed normal chow (Cholesterol: n = 22 APOE cKO and n = 19 littermate control mice; n = 14 D374Y and n = 19 littermate control mice. Triglycerides: n = 11 APOE cKO and n = 11 littermate control mice; n = 7 D374Y and n = 9 littermate control mice. PLs: n = 10 APOE cKO and n = 11 littermate control mice; n = 9 D374Y and n = 11 littermate control mice. Glycerol: n = 10 APOE cKO and n = 11 littermate control mice; n = 9 D374Y and n = 11 littermate control mice. FFA: n = 10 APOE cKO and n = 11 littermate control mice; n = 9 D374Y and n = 11 littermate control mice). b. Plasma lipoprotein fractionation profiles (µmol L−1) at 10 d after tamoxifen dosing. All curves were calculated as an average of two separately run plasma pools from male and female mice (plasma from 4–6 mice in each pool). c, Cholesterol and triglyceride measurements (µg mg−1 of protein) after liver Folch extraction from male and female mice (Cholesterol: n = 9 APOE cKO and n = 11 littermate control mice; n = 5 D374Y and n = 6 littermate control mice. Triglycerides: n = 9 APOE cKO and n = 11 littermate control mice; n = 5 D374Y and n = 6 littermate control mice). d, Representative pictures of liver section stained with ORO in APOE cKO and D374Y mice with respective littermate controls 10 d after dyslipidemia induction (scale bar, 100 μm). e, Circulating cholesterol and triglycerides (mg dl−1) measured in dyslipidemic APOE cKO, D374Y and respective littermate controls after 8 weeks on an HFD (Cholesterol: n = 5 APOE cKO and n = 3 littermate control mice; n = 5 D374Y and n = 3 littermate control mice. Triglycerides: n = 7 APOE cKO and n = 3 littermate control mice; n = 6 D374Y and n = 3 littermate control mice). f, Hepatic cholesterol and triglyceride levels measured as total liver cholesterol (mg) and total liver triglycerides (mg) in APOE cKO and D374Y with respective littermate controls (Cholesterol: n = 7 APOE cKO and n = 3 littermate control mice; n = 5 D374Y and n = 3 littermate control mice. Triglycerides: n = 7 APOE cKO and n = 3 littermate control mice; n = 5 D374Y and n = 3 littermate control mice). g, ORO representative liver sections of dyslipidemic APOE cKO and D374Y littermate control mice after 8 weeks on an HFD (scale bar, 100 μm). All plots are ±s.e.m. except 1a (±s.d.). For statistical analysis, a two-sided t-test was used (a,c,e,f). FFA, free fatty acid; VLDL, very-low-density lipoprotein.
