Fig. 1: Ischemic stroke and MI reduce plasma IgA levels and B cell follicle volumes in PP.
a, Concentrations of plasma IgA in patients with stroke and in healthy controls measured by ELISA (n = 14−23 per group, P = 0.0002). b,c, Concentrations of plasma IgA and fecal IgA in sham and stroke mice measured by ELISA (n = 6−8 per group, **P = 0.037, *P = 0.0053). d, Macroscopic overview of the mouse GI tract with the demarcation of PP 1 d after sham surgery or stroke. e, Fluorescence images after 3D reconstruction of stained PP showing the position of PP in the small intestine that were whole-mount stained with anti-CD19 (green) and anti-CD3 (blue) fluorescent antibodies before LSFM (left). Fluorescence single−channel images are shown (right); scale bar, 500 μm. f, Deep-learning-based automated analysis of B cell follicle volume in PP from duodenum, jejunum and ileum 1 d after stroke or sham (n = 7−11 PP per intestinal segment, 4−6 mice per group, sham versus stroke duodenum non-significant P > 0.9999, ***P = 0.0003, ****P < 0.0001). g, Concentrations of plasma IgA in patients with MI and healthy controls measured by ELISA (n = 16−39 per group, ****P < 0.0001). h, Deep-learning-based quantification of B cell follicle volume in PP of the duodenum, jejunum and ileum 1 d after sham or myocardial infarction (n = 4−9 PP per intestinal segment, 4−5 mice per group, sham versus MI non-significant P = 0.7879, ***P = 0.0002, *P = 0.0451). Data are mean ± s.d.; statistical analyses were performed by two-tailed Mann−Whitney U-test. All mouse data were combined from at least three independent experiments. HC, healthy control.
