Extended Data Fig. 2: Stroke and myocardial infarction induce shrinkage of B cell follicles and T cell zone volumes in PP.

a, 3D reconstruction images of CD19⁺ B cells and CD3⁺ T cells in PP isolated from duodenum, jejunum and ileum 24 h after stroke or sham surgery, scale bar=500 µm. b, The quantification of T cell zone volume in different intestinal segments after 24 h of stroke or sham controls (n = 8−11 PP per intestinal segment, two−tailed Mann−Whitney U test, duodenum sham vs stroke P > 0.9999, jejunum sham vs stroke P = 0.8633, leum sham vs stroke ***P = 0.0002). c, Numbers of GL7⁺ GC cells in PP after 24 h of sham or stroke operation using flow cytometry (n = 4 mice per group, unpaired t-test ***P = 0.0007). d, Representative fluorescence histology images of ileal PP stained with, anti−GL7, anti−EpCAM and DAPI after 24 h of stroke and sham operation, scale bar=200 µm. e, 3D reconstruction images of CD19⁺ B cells and CD3⁺ T cells in PP isolated from duodenum, jejunum and ileum 24 h after MI or sham surgery, scale bar=500 µm. f, The quantification of T cell zone volume in different intestinal segments after 24 h of myocardial infarction or sham controls (n = 4−6 PP per intestinal segment, two−tailed Mann−Whitney U test, duodenum sham vs MI P = 0.1636, jejunum sham vs MI *P = 0.0274, ileum sham vs MI P = 0.8329). g, 3D reconstruction images of CD19⁺ B cells and CD3⁺ T cells in PP isolated from ileum of naïve wild type, anti-CD20 treated wild type and J_hT^−/− mice, scale bar=500 µm. h, Quantification of the number of B cell follicles per PP in different intestinal regions after stroke or sham operation (n = 6 PP per group, two−tailed Mann−Whitney U test, duodenum sham vs stroke P = 0.6993, jejunum sham vs stroke P = 0.2456, ileum sham vs stroke P = 0.4082). Data represented as mean ±s.d., statistical analyses were performed by two−tailed Mann−Whitney U test. All data are combined from at least three independent experiments. PP=Peyer´s patches, MI=myocardial infarction.