Extended Data Fig. 7: Neutrophils after MI release NETs and induce loss of plasma cells and IgA.

a, Numbers of CD19⁺ and CD3⁺ cells in all PP in MI + vehicle and MI + DNase-I treated mice (n = 4−5 per group, unpaired t-test, **P = 0.0006, **P = 0.0035). b, Numbers of IgD⁻IgA⁺ CD138⁻ IgA-switched B cells in all PP and SI LP in MI and MI + DNase-I treated mice (n = 4−5 mice per group, unpaired t-test ***P = 0.0006, ****P < 0.0001). c, Numbers of IgD⁻IgA⁺ CD138⁺ plasma cells in all PP and SI LP in MI and MI + DNase-I treated mice (n = 4−5 mice per group, unpaired t-test, **P = 0.0024, ***P = 0.0005). d, Relative concentrations of plasma IgA in MI and MI + DNase-I treated mice (n = 4−5 per group, unpaired t-test ***P = 0.0003). e, Numbers of CD19⁺ and CD3⁺ cells in all PP in MI + isotype antibody and MI + anti-Ly6G treated mice (n = 4−6 per group, unpaired t-test, CD19⁺ **P = 0.0031, CD3⁺ **P = 0.0047). f, Numbers of IgD⁻IgA⁺CD138⁻ B cells in all PP and SI LP in MI + isotype antibody and MI + anti-Ly6G treated mice (n = 4−5 mice per group, unpaired t-test, ****P < 0.0001). g, Numbers of IgD⁻IgA⁺ CD138⁺ plasma cells in all PP and SI LP in MI + isotype antibody and MI + anti-Ly6G treated mice (n = 5−6 mice per group, unpaired t-test, ****P < 0.0001, ***P = 0.0006). h, Relative concentrations of plasma IgA in MI + isotype antibody and MI + anti-Ly6G treated mice (n = 4−5 per group, unpaired t-test **P = 0.0065). For B-D and F-H, data are presented as a percentage change and normalized to the mean of MI controls. Data represent mean ±s.d. All data is combined from at least two independent experiments, PP=Peyer’s patches, SI LP= small intestine lamina propria.