Extended Data Fig. 4: Baseline heart rate correlates with the effect of hESC-macrophages on the heart rate of cardiac microtissues.
(a) Microtissues (HT-Biowires) were seeded with hESC-cardiomyocytes and fibroblasts with or without hESC-macrophages. Active force was measured on day 14 post-seeding. n = 26 (control) or n = 24 (hESC-macrophage) microtissues per group pooled from two independent experiments. Unpaired t-test was performed. (b) Relationship between the baseline heart rate of microtissues (HT-Biowires, except for iCell data point from Biowire II platform) without hESC-macrophages to the change in heart rate upon addition of hESC-macrophages. Each data point represents the average of a distinct experiment, with the batch of cardiomyocytes used indicated. Simple linear regression was performed, reporting R-squared and P value indicating whether the slope is significantly non-zero. (c) Fold-change in active force in microtissues (HT-Biowires, except for iCell data from Biowire II platform) with hESC-macrophages relative to controls in each experiment performed. n = 14, 20, 6, 9, 11, 2, 6, 18, 6 control microtissues (left to right) or n = 8, 30, 5, 11, 14, 9, 16, 18, 6 microtissues with hESC-macrophages (left to right). Each group represents an independent experiment. CM: cardiomyocyte; FB: fibroblast; MF: macrophage. Unpaired two-tailed t-test (A, C): *P < 0.05, **P < 0.01, ***P < 0.001. Error bars represent mean ± s.e.m.
