Extended Data Fig. 7: Myo+SD+ IgG alter cardiomyocyte respiration. | Nature Cardiovascular Research

Extended Data Fig. 7: Myo+SD+ IgG alter cardiomyocyte respiration.

From: An engineered human cardiac tissue model reveals contributions of systemic lupus erythematosus autoantibodies to myocardial injury

Extended Data Fig. 7

(a) Schematic of the effects of Myo+SD+ IgG on oxidative phosphorylation pathway in cardiomyocytes. Red and blue boxes indicate upregulated and downregulated genes in the cardiomyocyte population, respectively, in tissues cultured with Myo+SD+ IgG compared to tissues cultured with Myo- IgG. (b-c) Sample gating of MitoTracker Green+ hiPSC-CMs cultured with patient IgG. Pseudocolor scatter plots of (b) unstained hiPSC-CMs and (c) hiPSC-CMs cultured with IgG from Myo+SD+ patients and stained for Propidium Iodide (PI) and MitoTracker Green. (d) Histogram of MitoTracker+ hiPSC-CMs cultured with IgG from healthy (black), Myo- (orange), Myo+SD- (blue), or Myo+SD+ (red) patients. hiPSC-CMs were dissociated prior to staining and analysis.

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