Extended Data Fig. 4: Intravascular staining FACS of Arg1^tdT-CreERT2 mice 2 days after MI.

a, FACS gating strategy for intravascular staining in the heart of Arg1^tdt-CreERT2 mice 2 days after I/R. Mice were injected with CD45 antibody 5 minutes prior to tissue collection (CD45IV). Cells were gated for leukocytes (SSC-A vs CD45), single cells (SSC-W vs SSC-H), debris exclusion (SSC-A vs FSC-A), myeloid cells (SSC-A vs CD11b), neutrophil exclusion (SSC-A vs Ly6G) and monocytes/macrophages (Ly6C vs CD64). Intravascular (CD45IV⁺) and extravascular cells (CD45IV⁻) were identified (CD45 vs CD45IV). Arg1⁺ cells were identified via TDT expression in the CD45iv⁻ and CD45iv⁺ gate (CD45 vs Arg1 (TDT)). b, Representative FACS plots of the peripheral blood of Arg1^tdt-CreERT2 mice 2 days after I/R when performing intravascular staining.