Extended Data Fig. 8: Single cell RNA sequencing of ZsGr⁻ and ZsGr⁺ cells in Control>ZsGr and Hif1a>ZsGr syngeneic heart transplant mice.

a, FACS gating strategy for isolation of ZsGr⁻ and ZsGr⁺ monocytes and macrophages from Control>ZsGr and Hif1a>ZsGr mice 5 days after syngeneic heart transplant (HTX) for use in scRNAseq. b, ZsGr positive and negative controls and TDT positive and negative controls which were used to determine gating for a. c, Violin plots representing scRNAseq data post quality control. Cells with greater than 500 and less than 5000 genes (nFeature_RNA), read counts greater than 500 and less than 20,000 (nCount_RNA), and proportion of transcripts mapping to mitochondrial genes less than 10 percent (prompt) were used for downstream analysis. d, Annotated UMAP of ZsGr⁻ and ZsGr⁺ cells in Control>ZsGr and Hif1a>Zsgr conditions 5 days after HTX. Arg1 expression plotted on a UMAP projection. e, Violin plot of Arg1 expression split between ZsGr⁻ and ZsGr⁺ cells in Control>ZsGr and Hifa1>ZsGr mice. f, Z-score profiles from of each subpopulation in the Control>ZsGr and Hifa1>ZsGr data set represented as a dot-plot. Z-score profile genes for each subpopulation are listed. g, Annotated UMAP of scRNAseq data 5 days after HTX split between ZsGr⁻ and ZsGr⁺ cells in Control>ZsGr and Hifa1>ZsGr mice, and stacked bar graph representing the proportion of each cell population in the data set split between conditions.