Extended Data Fig. 9: Palantir analysis of ZsGr⁻ and ZsGr⁺ cells in Control>ZsGr and Hif1a>ZsGr syngeneic heart transplant mice.

a, PCA plot of cell cycle genes in ZsGr^- and ZsGr⁺ cells in Control>ZsGr and Hif1a>ZsGr 5 days after syngeneic heart transplant (HTX) before (Cell Cycle PCA) and after (Post Correction) regressing out cell cycle scores. b, UMAP projection of scRNAseq data 5 days after HTX highlighting the starting cell used. Starting cell was determined by selecting the monocyte with the highest monocyte Z-score (Plac8, Ly6c2). c, Force directed layout (FDL) output of Palantir analysis highlighting each cell subpopulation in the ZsGr^- and ZsGr⁺ cells in Control>ZsGr and Hif1a>ZsGr 5 days after HTX. d, Plots of entropy vs pseudotime scores from Palantir analysis for every cell subpopulation in the scRNAseq data set.