Extended Data Fig. 1: GPNMB expression in the infarcted heart using immunofluorescent staining and flow cytometry.

a, Immunofluorescent staining, and confocal microscopy of GPNMB expression in uninjured (remote) and injured regions of heart demonstrating that GPNMB (green, arrows) is not coexpressed with CD90.2, CD31, CD146 and NG2 markers (red, arrows) at Day 7 post infarction (Representative images, n=3 animals). b, Flow cytometry of nonmyocytes in the heart 7 days after MI demonstrating the expression of GPNMB in macrophages (CD45, Ly6C, CD68 and CD11b, n=5 animals). Data presented as mean±SEM.