Extended Data Fig. 5: Klf9 is required for cardiac functional adaptation to hemodynamic pressure overload.
From: Klf9 is essential for cardiac mitochondrial homeostasis
a, Ratios of hearts or lungs weight to tibia length (n = 6). b, qRT-PCR analysis of mRNA levels of ANF, BNP and β-MHC in hearts from Ctrl and Klf9-CKO mice treated with saline or ANGII (n = 6). c, Left, WGA staining of hearts from Ctrl and Klf9-CKO mice treated with saline or ANGII. Right, Quantitative data for cardiomyocyte CSA (n = 6). d, Left, Masson’s trichrome staining of hearts from Ctrl and Klf9-CKO mice treated with saline or ANGII. Right, Quantification of blue-staining replacement fibrosis (n = 6). e, Left, TUNEL staining of hearts from Ctrl and Klf9-CKO mice treated with saline or ANGII. Right, Quantitative data for apoptosis positive cells (n = 6). f, qRT-PCR analysis of mRNA levels of genes involved in mitochondrial energy metabolism in hearts from above mice (n = 6). Data are shown as mean ± SEM; 2-way ANOVA (a, b, c, d, e).
