Extended Data Fig. 7: Klf9 regulates ANGII induced hypertrophy of NRCMs.
From: Klf9 is essential for cardiac mitochondrial homeostasis
a, Confocal imaging of NRCMs infected with Ad-shCtrl or Ad-shKlf9 for 24 hours, followed by treatment with ANGII (1 μM) or PBS for 48 hours. α-Actinin staining was performed to identify cells. b, Quantification of cell size of 30 NRCMs in each group (n = 22). c, Quantification of myocardial ATP levels in NRCMs as described in B (n = 12, n value refers to independent experiments). d, qRT-PCR analysis of mRNA levels of hypertrophic genes in NRCMs in A (n = 6, n value refers to independent experiments). e, Confocal imaging of NRCMs infected with Ad-GFP or Ad-Klf9 for 24 hours, followed by treatment with ANGII (1 μM) or PBS for 48 hours. α-Actinin staining was performed to identify cells. f, Quantification of cell size of 30 NRCMs in each group (n = 22). g, Quantification of myocardial ATP levels of NRCMs infected with Ad-GFP or Ad-Klf9, followed by treatment with ANGII or PBS (n = 12, n value refers to independent experiments). h, qRT-PCR analysis of mRNA levels of hypertrophic genes in NRCMs in E (n = 6, n value refers to independent experiments). Data are shown as mean ± SEM; 2-way ANOVA (b, c, d, f, g, h).
