Extended Data Fig. 9: Analysis of LEC proliferation.
From: Direct specification of lymphatic endothelium from mesenchymal progenitors
(a) ForceAtlas2 (FA) embedding of 19,699 cells based on partition-based graph abstraction (PAGA), with each dot representing a single cell. (b) FA embedding showing cell cycle phase in each cellular state. (c) Expression of selected cell cycle-related genes in single cells in angioblast, venous, LEC progenitor and lymphatic states. (d) Dual-pulse labelling strategy for analysis of cell proliferation. (e) Representative immunofluorescence image of ERG and KI67 expression in a transverse vibratome section from an E10.5 embryo (n = 3). (f) Quantification of growth fraction for ECs present inside the CV or DA. (CV, cardinal vein; DA, dorsal aorta; Scale bars - 25 μm (d); Data are presented as mean ± s.d. (error bars)).
