Extended Data Fig. 6: Deleting cloche/npas4l promotes myocardial migration in hand2 mutants.
From: PDGFRA is a conserved HAND2 effector during early cardiac development
a, In situ hybridization showing myl7 expression in 24 hpf hand2 FLD+/+, hand2 FLD−/−, npas4l−/−, and hand2 FLD−/−; npas4l−/− sibling embryos. b, In situ hybridization showing fn1a expression in 20 hpf hand2 FLD+/+, hand2 FLD−/−, npas4l−/−, and hand2 FLD−/−; npas4l−/− sibling embryos. To prevent overstaining hand2 FLD−/− and npas4l−/− embryos, we stopped staining reaction once some embryos became dark. Consequently, hand2 FLD+/+ embryos appear lighter in color. c, Normalized read count for fn1a expression in kdrl:mCherry+ endothelium from 20 hpf hand2 FLD+/? and hand2 FLD−/− sibling embryos. P value in c was calculated using an unpaired Student’s t-Test. Error bars are mean ± s.e.m.; n = 3 biologically independent samples. The proportion of embryos matching the image shown is indicated in the top right corner of each image. Scale bars apply to all images.
