Extended Data Fig. 9: 3xFLAG-Hand2 pull down experiment.
From: PDGFRA is a conserved HAND2 effector during early cardiac development
a, FLAG IP of lysates from 14 hpf 3xFLAG-hand2 and 3xFLAG-hand2 EDE mRNA injected embryos blotted with antibodies against FLAG and β-actin. b, b’, Absolute protein intensity of Hand2, Tcf3a, and Tcf3b in 3xFLAG-Hand2 and 3xFLAG-Hand2 EDE protein complexes by mass spectrometry; error bars are mean ± s.d.; n = 3 biologically independent samples; list of the mean of absolute protein intensity shown in b’.
