Extended Data Fig. 2: Generation of hand2 mutant alleles.
From: PDGFRA is a conserved HAND2 effector during early cardiac development
a, Amino acid sequence alignment of Human HAND2, mouse HAND2, and zebrafish Hand2 generated through Clustal Omega. The three arginines in red have DNA binding activity; the threonine and serine in blue have phosphorylation activity; the phenylalanine in green has dimerization activity. While there is 100% amino acid sequence identity between human and mouse HAND2, the residues that are different in zebrafish are indicated in orange. b, Partial nucleotide sequence of the hand2 FLD allele, which shows a 1,411 bp deletion, the hand2 Δ27 allele, and the hand2 Δ3 allele. c, Brightfield images of 75 hpf hand2 FLD+/+ and hand2 FLD−/− sibling larvae, hand2 Δ27+/+ and hand2 Δ27−/− sibling larvae, hand2 Δ3+/+ and hand2 Δ3−/− sibling larvae, hand2 Δ27/FLD transheterozygous larvae, and hand2 Δ27/Δ3 transheterozygous larvae. The proportion of larvae matching the image shown is indicated in the top right corner of each image. Scale bars apply to all images.
