Extended Data Fig. 3: Activation of PKN1 by disturbed flow.
a, HUAECs were exposed to disturbed flow (4 dynes/cm2) for 24 hours. PKN1 phosphorylation was determined by immunoblotting using an antibody directed against phosphorylated PKN1. The bar diagram shows the statistical analysis (n = 4 independent experiments). b,c, HUAECs were exposed to disturbed flow for the indicated time periods. Thereafter, phosphorylation of PKN1 (b) or PKN2 (c) was determined by immunoblotting using phosphosite-specific antibodies (n = 3 independent experiments). d, HUAECs were exposed to disturbed flow (4 dynes/cm2) for 24 hours. PKN1 localization was determined by immunofluorescence staining using an antibody directed against PKN1 (green) and with DAPI (blue). The bar diagram shows the statistical analysis (n = 4 independent experiments). Data are shown as mean ± s.e.m.; the p-values are given in the figure (Mann-Whitney two-sided test (a, d), Kruskal-Wallis test (b, c)).
